Olfactory nerve stimulation-evoked mGluR1 slow potentials, oscillations, and calcium signaling in mouse olfactory bulb mitral cells

J Neurophysiol. 2006 May;95(5):3097-104. doi: 10.1152/jn.00001.2006. Epub 2006 Feb 8.

Abstract

Fast synaptic transmission between olfactory receptor neurons and mitral cells (MCs) is mediated through AMPA and NMDA ionotropic glutamate receptors. MCs also express high levels of metabotropic glutamate receptor 1 (mGluR1) whose functional significance is less understood. Here we characterized a slow mGluR1-mediated potential that was evoked by high-frequency (100-Hz) olfactory nerve (ON) stimulation in the presence of NBQX and D-APV, blockers of ionotropic glutamate receptors, and that was associated with a local Ca2+ transient in the MC dendritic tuft. High-frequency ON stimulation in the presence of NBQX and D-APV also evoked a slow, nearly 2-Hz oscillation of MC membrane potential that was abolished by the mGluR1 antagonist LY367385 (50 microM). Both mGluR slow potential and slow oscillation persisted in the presence of gabazine (10 microM), a GABA(A) receptor antagonist, and intracellular QX-314 (10 mM), a Na+ channel blocker. In contrast to a slow mGluR1 potential in cerebellar Purkinje neurons, the MC mGluR1 potential was not depressed by SKF96365 (< or =250 microM) and thus is likely not mediated by TRPC1 cation channels, nor was it potentiated by an elevation of intracellular Ca2+ level. Imaging with the Na+ indicator SBFI revealed a Na+ transient in the MC dendrite accompanying the mGluR1 slow potential. We conclude that the MC mGluR1 potential triggered by glutamate released from the ON supports oscillations and synchronizations of MCs associated within one glomerulus.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Animals, Newborn
  • Aspartic Acid / pharmacology
  • Calcium Channel Blockers / pharmacology
  • Calcium Signaling / radiation effects*
  • Diagnostic Imaging / methods
  • Dose-Response Relationship, Radiation
  • Drug Interactions
  • Electric Stimulation / methods
  • Evoked Potentials / physiology
  • Evoked Potentials / radiation effects*
  • Excitatory Amino Acid Antagonists / pharmacology
  • GABA Antagonists / pharmacology
  • Glycine / analogs & derivatives
  • Glycine / pharmacology
  • Imidazoles / pharmacology
  • In Vitro Techniques
  • Lidocaine / analogs & derivatives
  • Lidocaine / pharmacology
  • Mice
  • Mice, Inbred ICR
  • Neurons / physiology
  • Neurons / radiation effects*
  • Olfactory Bulb / cytology*
  • Olfactory Nerve / radiation effects*
  • Pyridazines / pharmacology
  • Receptors, Metabotropic Glutamate / antagonists & inhibitors
  • Receptors, Metabotropic Glutamate / metabolism*
  • Sodium Channel Blockers / pharmacology

Substances

  • Calcium Channel Blockers
  • Excitatory Amino Acid Antagonists
  • GABA Antagonists
  • Imidazoles
  • Pyridazines
  • Receptors, Metabotropic Glutamate
  • Sodium Channel Blockers
  • benzyloxyaspartate
  • metabotropic glutamate receptor type 1
  • methyl-(4-carboxyphenyl)glycine
  • QX-314
  • Aspartic Acid
  • Lidocaine
  • gabazine
  • 1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
  • Glycine