Identification of integrin alpha1 as an interacting protein of protein tyrosine phosphatase PRL-3

Biochem Biophys Res Commun. 2006 Mar 31;342(1):179-83. doi: 10.1016/j.bbrc.2006.01.102. Epub 2006 Feb 2.


PRL-3 is a newly identified protein tyrosine phosphatase associated with tumor metastasis. It is over-expressed in various cancers, such as colorectal cancer, gastric cancer, and ovarian cancer, and is correlated with the progression and survival of cancers. Although PRL-3 plays a causative role in promoting cancer cell invasion and metastasis, the molecular mechanism is unknown. To investigate PRL-3's roles in tumorigenesis and signal transduction pathway, we screened the human placenta brain cDNA library with the bait of PRL-3 in yeast two-hybrid system. Then we identified integrin alpha1 as a PRL-3-interacting protein for the first time, and verified this physical association with pull-down and co-immunoprecipitation assays. Furthermore, we found that PRL-3 could down-regulate the tyrosine-phosphorylation level of integrin beta1 and increased the phosphorylation level of Erk1/2. Our present discovery will provide new clues for elucidating the molecular mechanism of PRL-3 in promoting cancer invasion and metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Down-Regulation
  • Humans
  • Immediate-Early Proteins / genetics
  • Immediate-Early Proteins / metabolism*
  • Integrin alpha1 / chemistry
  • Integrin alpha1 / genetics
  • Integrin alpha1 / isolation & purification
  • Integrin alpha1 / metabolism*
  • Integrin beta Chains / chemistry
  • Integrin beta Chains / metabolism
  • Mitogen-Activated Protein Kinase 1 / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism*
  • Phosphorylation
  • Phosphotyrosine / metabolism
  • Protein Binding
  • Protein Tyrosine Phosphatases / genetics
  • Protein Tyrosine Phosphatases / metabolism*


  • Immediate-Early Proteins
  • Integrin alpha1
  • Integrin beta Chains
  • Neoplasm Proteins
  • Phosphotyrosine
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • PTP4A3 protein, human
  • Protein Tyrosine Phosphatases