MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis

Int J Legal Med. 2006 May;120(3):174-81. doi: 10.1007/s00414-005-0074-6. Epub 2006 Feb 11.


We sought to evaluate a more effective system for analyzing X-chromosomal short tandem repeats (X-STRs) in highly degraded DNA. To generate smaller amplicon lengths, we designed new polymerase chain reaction (PCR) primers for DXS7423, DXS6789, DXS101, GATA31E08, DXS8378, DXS7133, DXS7424, and GATA165B12 at X-linked short tandem repeat (STR) loci, devising two miniX-multiplex PCR systems. Among 333 Japanese individuals, these X-linked loci were detected in amplification products ranging in length from 76 to 169 bp, and statistical analyses of the eight loci indicated a high usefulness for the Japanese forensic practice. Results of tests on highly degraded DNA indicated the miniX-STR multiplex strategies to be an effective system for analyzing degraded DNA. We conclude that analysis by the current miniX-STR multiplex systems offers high effectiveness for personal identification from degraded DNA samples.

MeSH terms

  • Asian People / genetics
  • Chromosomes, Human, X*
  • DNA Degradation, Necrotic*
  • DNA Fingerprinting
  • DNA Primers
  • Female
  • Gene Frequency
  • Genetics, Population*
  • Humans
  • Japan
  • Male
  • Minisatellite Repeats*
  • Polymerase Chain Reaction


  • DNA Primers