C-type natriuretic peptide is a growth inhibitor of rat vascular smooth muscle cells

Biochem Biophys Res Commun. 1991 Jun 28;177(3):927-31. doi: 10.1016/0006-291x(91)90627-j.


C-type natriuretic peptide (CNP) which potently stimulates particulate guanylate cyclase activity in cultured rat vascular smooth muscle cells (VSMC) inhibited serum-induced DNA synthesis of the cells 10-fold more effectively than alpha-human atrial natriuretic peptide (alpha-hANP). The inhibitory effect of CNP was mimicked by 8-bromo-cGMP. The proliferation of VSMC was also suppressed by CNP more potently than alpha-hANP, while the peptide was less active for cGMP augmentation and for vasorelaxation than alpha-hANP in isolated rat aorta. These results suggest that CNP may be a growth regulating factor of VSMC rather than a vasodilator.

MeSH terms

  • Animals
  • Aorta, Thoracic / cytology
  • Aorta, Thoracic / drug effects
  • Aorta, Thoracic / physiology*
  • Atrial Natriuretic Factor / pharmacology*
  • Cell Division / drug effects
  • Cells, Cultured
  • Culture Media
  • Cyclic GMP / metabolism
  • DNA Replication / drug effects
  • Growth Inhibitors / pharmacology*
  • Humans
  • Kinetics
  • Male
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / physiology*
  • Natriuretic Peptide, C-Type
  • Nerve Tissue Proteins / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Thymidine / metabolism


  • Culture Media
  • Growth Inhibitors
  • Nerve Tissue Proteins
  • Natriuretic Peptide, C-Type
  • Atrial Natriuretic Factor
  • Cyclic GMP
  • Thymidine