Regulation of surface CD163 expression and cellular effects of receptor mediated hemoglobin-haptoglobin uptake on human monocytes and macrophages

Cytometry A. 2006 Mar;69(3):203-5. doi: 10.1002/cyto.a.20235.

Abstract

Local dysregulation of iron metabolism is suggested to contribute to atherosclerotic lesion development through hemoglobin scavenging pathways. We evaluated the effects of CD163-mediated uptake of hemoglobin-haptoglobin (HbHp) complexes on surface CD163 and intracellular heme oxygenase-1 expression and the secretion of pro- and antiinflammatory cytokines by macrophages. We found that increased availability of HbHp complexes triggers the upregulation of surface CD163, and also results in a dose-dependent secretion of IL-6 and IL-10.

MeSH terms

  • Antigens, CD / metabolism*
  • Antigens, Differentiation, Myelomonocytic / metabolism*
  • Flow Cytometry
  • Gene Expression / drug effects
  • Gene Expression / genetics
  • Haptoglobins / pharmacokinetics*
  • Haptoglobins / pharmacology
  • Heme Oxygenase-1 / genetics
  • Heme Oxygenase-1 / metabolism
  • Hemoglobins / pharmacokinetics*
  • Hemoglobins / pharmacology
  • Humans
  • Interleukin-10 / metabolism
  • Interleukin-6 / metabolism
  • Interleukins / metabolism*
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / metabolism*

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • Haptoglobins
  • Hemoglobins
  • Interleukin-6
  • Interleukins
  • RNA, Messenger
  • Receptors, Cell Surface
  • haptoglobin-hemoglobin complex
  • Interleukin-10
  • HMOX1 protein, human
  • Heme Oxygenase-1