Isolation of type I and type II GABAA-benzodiazepine receptors by immunoaffinity chromatography

FEBS Lett. 1991 Jun 17;284(1):15-8. doi: 10.1016/0014-5793(91)80750-w.

Abstract

Anti-peptide alpha 1 (1-9) and anti-peptide alpha 3 (459-467) antibodies coupled to Affigel-10 were used for the isolation of GABAA receptors containing the alpha 1- or alpha 3-subunit, respectively. Both types of GABAA receptors exhibited a high affinity for [3H]flunitrazepam, and binding of [3H]flunitrazepam was stimulated in the presence of GABA. GABAA receptors eluted from the anti-peptide alpha 1 (1-9) immunoaffinity column exhibited a high affinity and those from the anti-peptide alpha 3 (459-467) columns a low affinity for the type I benzodiazepine receptor-selective ligand Cl 218872, indicating the enrichment of type I and type II GABAA-benzodiazepine receptors, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / immunology
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Peptide Fragments / immunology
  • Rats
  • Receptors, GABA-A / immunology
  • Receptors, GABA-A / isolation & purification*

Substances

  • Peptide Fragments
  • Receptors, GABA-A