Loss of anti-aggregatory effects of aortic valve tissue in patients with aortic stenosis

J Heart Valve Dis. 2006 Jan;15(1):28-33.


Background and aim of the study: Patients with aortic stenosis (AS) exhibit increased platelet aggregability, and thrombus formation has been documented on calcific and severely stenosed valves. Isolated porcine and canine aortic valves (AV) release nitric oxide (NO) and prostacyclin, which exert local antithrombotic effects; to date, this has not been studied in humans. In the present study the possible interaction of AV tissue with platelet aggregation was examined, using fragments of AV obtained from patients with AS and aortic regurgitation (AR).

Methods: Fragments of AV tissue, excised from patients undergoing AV replacement, were co-incubated with blood samples obtained from normal subjects. The direct effects of valve tissue from patients with AS (n = 14) or with predominant AR (n = 13) on ADP-induced platelet aggregation and intraplatelet cGMP and cAMP content were compared.

Results: In whole blood, non-calcified AV fragments from AR patients inhibited platelet aggregation by 57 +/- 6% (p < 0.01); in platelet-rich plasma results were analogous. In order to determine whether this anti-aggregatory effect could be attributed to the valvular release of NO or prostacyclin, intraplatelet cGMP and cAMP formation was assessed, respectively. While there were no significant changes in cGMP content, cAMP increased by 26 +/- 4% (p < 0.02). Both, anti-aggregatory and cAMP-stimulating effects were similar to those produced by 10 nM prostaglandin E1, a prostacyclin mimetic. Fragments from stenotic valves did not inhibit aggregation and did not affect cGMP or cAMP. Furthermore, fragments from heavily calcified regions potentiated aggregation and, in some cases, induced spontaneous aggregation.

Conclusion: Minimally calcified aortic valves (i.e., AR) and, therefore, presumably also normal valves, exert anti-aggregatory effects, most likely via prostacyclin release. AS is associated with a loss of this effect, thus potentially contributing to thrombotic risk.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / administration & dosage
  • Aged
  • Alprostadil / administration & dosage
  • Aortic Valve / physiopathology*
  • Aortic Valve Insufficiency / physiopathology
  • Aortic Valve Stenosis / blood
  • Aortic Valve Stenosis / physiopathology*
  • Biomarkers / blood
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism
  • Cyclic AMP / blood
  • Cyclic GMP / blood
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism
  • Epoprostenol / blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nitric Oxide / blood
  • Platelet Aggregation Inhibitors / administration & dosage
  • Platelet Aggregation* / drug effects
  • Research Design


  • Biomarkers
  • Platelet Aggregation Inhibitors
  • Nitric Oxide
  • Adenosine Diphosphate
  • Epoprostenol
  • Cyclic AMP
  • Alprostadil
  • Cyclic GMP