Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG

FEBS Lett. 2006 Mar 6;580(6):1547-52. doi: 10.1016/j.febslet.2006.01.082. Epub 2006 Feb 2.

Abstract

In the methylerythritol phosphate pathway for isoprenoid biosynthesis, the GcpE/IspG enzyme catalyzes the conversion of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate. This reaction requires a double one-electron transfer involving a [4Fe-4S] cluster. A thylakoid preparation from spinach chloroplasts was capable in the presence of light to act as sole electron donor for the plant GcpE Arabidopsis thaliana in the absence of any pyridine nucleotide. This is in sharp contrast with the bacterial Escherichia coli GcpE, which requires flavodoxin/flavodoxin reductase and NADPH as reducing system and represents the first proof that the electron flow from photosynthesis can directly act in phototrophic organisms as reducer in the 2-C-methyl-d-erythritol 4-phosphate pathway, most probably via ferredoxin, in the absence of any reducing cofactor. In the dark, the plant GcpE catalysis requires in addition of ferredoxin NADP(+)/ferredoxin oxido-reductase and NADPH as electron shuttle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / metabolism
  • Arabidopsis / enzymology
  • Arabidopsis Proteins / isolation & purification
  • Arabidopsis Proteins / metabolism*
  • Electron Transport
  • Erythritol / analogs & derivatives
  • Erythritol / metabolism
  • Escherichia coli / enzymology
  • Ferredoxins / metabolism*
  • Light
  • Oxidation-Reduction
  • Oxidoreductases
  • Photosynthesis*
  • Spinacia oleracea / enzymology
  • Spinacia oleracea / metabolism*
  • Spinacia oleracea / radiation effects
  • Terpenes / metabolism*
  • Thylakoids / enzymology
  • Thylakoids / metabolism*
  • Thylakoids / radiation effects

Substances

  • Arabidopsis Proteins
  • Ferredoxins
  • Terpenes
  • methyl-D-erythritol 2,4-cyclodiphosphate
  • Oxidoreductases
  • At5g60600 protein, Arabidopsis
  • Alkyl and Aryl Transferases
  • Erythritol