Investigation of the mechanism of action of 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans by flow cytometry

Bioorg Med Chem Lett. 2006 May 1;16(9):2492-5. doi: 10.1016/j.bmcl.2006.01.094. Epub 2006 Feb 15.

Abstract

The mechanism of action of the antifungal agent 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans was investigated by flow cytometry, using propidium iodide, DiBAC4(3), and FUN-1 as the fluorescent dyes. A related but less active agent, together with amphotericin B and fluconazole, was tested in parallel for comparison of the results. The incrustoporine derivative was found to have a potent fungicidal activity on C. albicans, resulting in damage of cell membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Candida albicans / drug effects*
  • Flow Cytometry / methods*
  • Furans / chemistry
  • Furans / pharmacology*
  • Microbial Sensitivity Tests
  • Structure-Activity Relationship

Substances

  • 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one
  • Antifungal Agents
  • Furans