Expression of p53, p16 and COX-2 in pancreatic cancer with tissue microarray

Hepatobiliary Pancreat Dis Int. 2006 Feb;5(1):138-42.


Background: Pancreatic cancer development and progression is driven by the accumulation of genetic changes. In this study we constructed tissue microarray containing specimens from pancreatic cancer, adjacent non-cancer tissue and normal tissue to survey the expression of p53, p16 and cyclooxygenase-2 (COX-2).

Methods: Tissue microarray containing 337 specimens from different stages of pancreatic cancer, adjacent non-cancer tissue and normal tissues was constructed, and the expression of p53, p16 and COX-2 was assayed by immunohistochemistry to consecutive formalin-fixed tissue microarray sections.

Results: The expression of p53, p16 and COX-2 was significantly higher in tumorous tissues than in non-tumorous ones. A significant relationship was observed between p53 and COX-2, or p16 and COX-2. But no obvious correlation was seen between p53 and p16 expressions. Logistic regression analysis showed p53 and COX-2 as dependent predictors in pancreatic carcinogenesis, and a reciprocal relationship to neoplastic progression between p53 and COX-2.

Conclusion: Combination analysis of p53 and COX-2 may be useful in predicting pancreatic carcinogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biopsy
  • Child
  • Cyclooxygenase 2 / biosynthesis
  • Cyclooxygenase 2 / genetics*
  • DNA, Neoplasm / biosynthesis
  • DNA, Neoplasm / genetics*
  • Disease Progression
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Genes, p16 / physiology*
  • Genes, p53 / genetics*
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology
  • Retrospective Studies
  • Tissue Array Analysis


  • DNA, Neoplasm
  • Cyclooxygenase 2