Alcohol potently modulates climbing fiber-->Purkinje neuron synapses: role of metabotropic glutamate receptors

J Neurosci. 2006 Feb 15;26(7):1906-12. doi: 10.1523/JNEUROSCI.4430-05.2006.


Consumption of alcoholic beverages produces alterations in motor coordination and equilibrium that are responsible for millions of accidental deaths. Studies indicate that ethanol produces these alterations by affecting the cerebellum, a brain region involved in the control of motor systems. Purkinje neurons of the cerebellar cortex have been shown to be particularly important targets of ethanol. However, its mechanism of action at these neurons is poorly understood. We hypothesized that ethanol could modulate Purkinje neuron function by altering the excitatory input provided by the climbing fiber from the inferior olive, which evokes a powerful all-or-none response denoted as the complex spike. To test this hypothesis, we performed whole-cell patch-clamp electrophysiological and Ca2+ imaging experiments in acute slices from rat cerebella. We found that ethanol potently inhibits the late phase of the complex spike and that this effect is the result of inhibition of type-1 metabotropic glutamate receptor-dependent responses at the postsynaptic level. Moreover, ethanol inhibited climbing fiber long-term depression, a form of synaptic plasticity that also depends on activation of these metabotropic receptors. Our findings identify the climbing fiber-->Purkinje neuron synapse as an important target of ethanol in the cerebellar cortex and indicate that ethanol significantly affects cerebellar circuits even at concentrations as low as 10 mm (legal blood alcohol level in the United States is below 0.08 g/dl = 17 mm).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alanine / analogs & derivatives
  • Alanine / pharmacology
  • Animals
  • Aspartic Acid / pharmacology
  • Disease Models, Animal
  • Ethanol / pharmacology*
  • Long-Term Synaptic Depression / drug effects
  • Male
  • Nerve Fibers / drug effects
  • Nerve Fibers / physiology*
  • Patch-Clamp Techniques
  • Purkinje Cells / drug effects
  • Purkinje Cells / physiology*
  • Quinoxalines / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Metabotropic Glutamate / drug effects
  • Receptors, Metabotropic Glutamate / physiology*
  • Synapses / drug effects
  • Synapses / physiology*
  • Xanthenes / pharmacology


  • 2-amino-2-(3-carboxycyclobutyl)-3-(9H-xanthen-9-yl)propionic acid
  • Quinoxalines
  • Receptors, Metabotropic Glutamate
  • Xanthenes
  • benzyloxyaspartate
  • 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline
  • Aspartic Acid
  • Ethanol
  • Alanine