Molecular analysis and susceptibility profiling of Candida albicans isolates from immunocompromised patients in South India

Mycopathologia. 2006 Mar;161(3):153-9. doi: 10.1007/s11046-005-9210-6.

Abstract

The genetic diversity and in vitro antifungal susceptibility profiles of 55 Candida albicans from immunocompromised patients were studied. PCR based analysis of the transposable intron in the 25S rDNA revealed 39 genotype A, 4 genotype B and 12 genotype C isolates. Serotype analysis categorized 52 isolates as serotype A and 3 as serotype B. All strains were susceptible to micafungin, 5-flucytosine and miconazole, whereas resistance against amphotericin B (3.6%), fluconazole (3.6%), itraconazole (7.3%) and voriconazole (5.5%) was observed. No association was seen between antifungal resistance and genotype/serotype status.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Candida albicans / drug effects*
  • Candida albicans / genetics*
  • Candida albicans / isolation & purification
  • Candidiasis / immunology*
  • Candidiasis / microbiology*
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Genetic Variation
  • Genotype
  • Humans
  • Immunocompromised Host*
  • India
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • Serotyping

Substances

  • Antifungal Agents
  • DNA, Fungal
  • RNA, Ribosomal
  • RNA, ribosomal, 25S