IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

Prostate. 2006 Jun 1;66(8):789-800. doi: 10.1002/pros.20379.

Abstract

Background: IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases.

Methods: Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT.

Results: CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response.

Conclusions: IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bone Neoplasms / pathology
  • Bone Neoplasms / physiopathology
  • Bone Neoplasms / secondary*
  • Cell Communication
  • Cell Differentiation
  • Cell Line
  • Cell Line, Tumor
  • Cell Proliferation
  • Culture Media, Conditioned / chemistry
  • Humans
  • Insulin-Like Growth Factor Binding Protein 2 / pharmacology
  • Insulin-Like Growth Factor I / analysis
  • Insulin-Like Growth Factor I / metabolism*
  • Insulin-Like Growth Factor I / pharmacology
  • Insulin-Like Growth Factor I / physiology*
  • Male
  • Mice
  • Mice, SCID
  • Neoplasm Metastasis / physiopathology
  • Osteoblasts / cytology*
  • Osteoblasts / physiology
  • Osteolysis / physiopathology
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology*
  • Prostatic Neoplasms / physiopathology
  • Tibia

Substances

  • Culture Media, Conditioned
  • Insulin-Like Growth Factor Binding Protein 2
  • Insulin-Like Growth Factor I