Abstract
Aims:
Mounting evidence indicates that deregulation of apoptosis contributes to the development of human cancers. Bcl-2 family proteins regulate the intrinsic apoptosis pathway. The aim of this study was to explore the possibility that mutation of BH3 domain of proapoptotic Bcl-2 genes Bad, Bmf and Bcl-G might be involved in the development of urinary bladder cancer.
Methods:
We analysed the BH3 domains of Bad, Bmf and Bcl-G genes for the detection of somatic mutations in 43 transitional cell carcinomas (TCCs) of the urinary bladder by a single strand conformation polymorphism assay in this study.
Results:
There was no somatic mutation of BH3 domains of Bad, Bmf and Bcl-G genes in the TCC samples.
Conclusion:
The data presented here indicate that BH3 domain mutation of these genes is rare in TCCs and may not contribute to the pathogenesis of TCCs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / physiology
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Apoptosis / genetics*
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BH3 Interacting Domain Death Agonist Protein / genetics*
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BH3 Interacting Domain Death Agonist Protein / physiology
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Carcinoma, Transitional Cell / genetics*
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Carcinoma, Transitional Cell / physiopathology
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DNA, Neoplasm / genetics
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Humans
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Mutation / genetics
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational
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Proto-Oncogene Proteins c-bcl-2 / genetics*
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Proto-Oncogene Proteins c-bcl-2 / physiology
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Urinary Bladder Neoplasms / genetics*
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Urinary Bladder Neoplasms / physiopathology
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bcl-Associated Death Protein / genetics*
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bcl-Associated Death Protein / physiology
Substances
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Adaptor Proteins, Signal Transducing
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BAD protein, human
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BCL2L14 protein, human
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BH3 Interacting Domain Death Agonist Protein
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BID protein, human
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BMF protein, human
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DNA, Neoplasm
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Proto-Oncogene Proteins c-bcl-2
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bcl-Associated Death Protein