A Receptor Tyrosine Kinase Specific to Hematopoietic Stem and Progenitor Cell-Enriched Populations

Cell. 1991 Jun 28;65(7):1143-52. doi: 10.1016/0092-8674(91)90010-v.

Abstract

To elucidate the molecular biology of the hematopoietic stem cell, we have begun to isolate genes from murine cell populations enriched in stem cell activity. One such cDNA encodes a novel receptor tyrosine kinase, designated fetal liver kinase-2 or flk-2, which is related to the W locus gene product c-kit. Expression analyses suggest an extremely restricted distribution of flk-2. It is expressed in populations enriched for stem cells and primitive uncommitted progenitors, and is absent in populations containing more mature cells. Therefore, this receptor may be a key signal transducing component in the totipotent hematopoietic stem cell and its immediate self-renewing progeny.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Separation
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Hematopoiesis
  • Hematopoietic Stem Cells / enzymology
  • Hematopoietic Stem Cells / physiology*
  • Liver / embryology
  • Liver / physiology
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Protein-Tyrosine Kinases / genetics*
  • Proto-Oncogene Proteins*
  • RNA, Messenger / genetics
  • Receptor Protein-Tyrosine Kinases*
  • Receptors, Cell Surface / genetics*
  • Thymus Gland / cytology
  • Thymus Gland / physiology
  • fms-Like Tyrosine Kinase 3

Substances

  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • DNA
  • Flt3 protein, mouse
  • Protein-Tyrosine Kinases
  • Receptor Protein-Tyrosine Kinases
  • fms-Like Tyrosine Kinase 3

Associated data

  • GENBANK/M59201
  • GENBANK/M63700
  • GENBANK/M63702
  • GENBANK/M63718
  • GENBANK/M64689
  • GENBANK/S40354
  • GENBANK/S63145
  • GENBANK/S63146
  • GENBANK/S63147
  • GENBANK/S63148