The brain ryanodine receptor: a caffeine-sensitive calcium release channel

Neuron. 1991 Jul;7(1):17-25. doi: 10.1016/0896-6273(91)90070-g.

Abstract

The release of stored Ca2+ from intracellular pools triggers a variety of important neuronal processes. Physiological and pharmacological evidence has indicated the presence of caffeine-sensitive intracellular pools that are distinct from the well-characterized inositol 1,4,5,-trisphosphate (IP3)-gated pools. Here we report that the brain ryanodine receptor functions as a caffeine- and ryanodine-sensitive Ca2+ release channel that is distinct from the brain IP3 receptor. The brain ryanodine receptor has been purified 6700-fold with no change in [3H]ryanodine binding affinity and shown to be a homotetramer composed of an approximately 500 kd protein subunit, which is identified by anti-peptide antibodies against the skeletal and cardiac muscle ryanodine receptors. Our results demonstrate that the brain ryanodine receptor functions as a caffeine-sensitive Ca2+ release channel and thus is the likely gating mechanism for intracellular caffeine-sensitive Ca2+ pools in neurons.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biophysics / methods
  • Brain / metabolism*
  • Caffeine / pharmacology*
  • Calcium / metabolism*
  • Calcium Channels*
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates / metabolism
  • Lipid Bilayers
  • Microscopy, Electron / methods
  • Rabbits
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Cholinergic / isolation & purification
  • Receptors, Cholinergic / physiology*
  • Receptors, Cholinergic / ultrastructure
  • Receptors, Cytoplasmic and Nuclear*
  • Ryanodine Receptor Calcium Release Channel

Substances

  • Calcium Channels
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates
  • Lipid Bilayers
  • Receptors, Cell Surface
  • Receptors, Cholinergic
  • Receptors, Cytoplasmic and Nuclear
  • Ryanodine Receptor Calcium Release Channel
  • Caffeine
  • Calcium