Diagnostic accuracy of a bedside qualitative immunochromatographic test for acute myocardial infarction

Am J Emerg Med. 2006 Mar;24(2):149-55. doi: 10.1016/j.ajem.2005.08.002.


Objective: To evaluate the diagnostic efficacy of a bedside immunochromatographic test (CardioDetect) that identifies human heart-type fatty acid-binding protein in whole blood.

Methods: Sixty-four patients with chest pain had CardioDetect test performed together with serial determination of serum troponin I (TnI) and creatine kinase (CK). CardioDetect results were interpreted twice, 24 hours apart, by 3 independent observers who were blinded to patients' clinical and laboratory data. Acute myocardial infarction was diagnosed by a physician blinded to the results of the CardioDetect test. Sensitivity, specificity, positive and negative predictive values, diagnostic accuracy, and receiver operating characteristic curves were determined for all tests at 4 predefined times. Intraobserver and interobserver reliabilities were determined.

Results: At less than 4 hours after chest pain, CardioDetect had a sensitivity and specificity of 62.5% and 100% compared with 50% and 80% for TnI and 37.5% and 80% for CK, respectively. At more than 4 hours but 12 hours or less in duration, the sensitivity of CardioDetect and TnI was 100%, whereas that of CK was 85.7%. Intraobserver agreement and interobserver reliability were high for CardioDetect (kappa >0.80 [P < .0001] and intraclass correlation coefficient, >0.90, respectively).

Conclusion: CardioDetect was a reliable point-of-care test for the early diagnosis (<12 hours) of acute myocardial infarction in the ED.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Fatty Acid Binding Protein 3
  • Fatty Acid-Binding Proteins / blood*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Myocardial Infarction / blood
  • Myocardial Infarction / diagnosis*
  • Point-of-Care Systems*
  • Prospective Studies
  • ROC Curve
  • Sensitivity and Specificity
  • Time Factors


  • FABP3 protein, human
  • Fatty Acid Binding Protein 3
  • Fatty Acid-Binding Proteins