Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions and chemical reagents

Dermatology. 2006;212 Suppl 1(Suppl 1):119-23. doi: 10.1159/000089211.

Abstract

The efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10(6) TCID(50)/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56 degrees C for 60 min or longer reduced the infectivity of the virus from 2.6 x 10(7) to undetectable levels. Irradiation with ultraviolet light at 134 microW/cm(2) for 15 min reduced the infectivity from 3.8 x 10(7) to 180 TCID(50)/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID(50)/ml.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetone / pharmacology
  • Animals
  • Anti-Infective Agents, Local / pharmacology*
  • Antiviral Agents / pharmacology
  • Chlorocebus aethiops
  • Ethanol / pharmacology
  • Formaldehyde / pharmacology
  • Glutaral / pharmacology
  • Hot Temperature
  • Indicators and Reagents / pharmacology
  • Methanol / pharmacology
  • Povidone-Iodine / pharmacology*
  • SARS Virus / drug effects*
  • SARS Virus / radiation effects*
  • Ultraviolet Rays
  • Vero Cells
  • Virus Inactivation*

Substances

  • Anti-Infective Agents, Local
  • Antiviral Agents
  • Indicators and Reagents
  • Acetone
  • Formaldehyde
  • Ethanol
  • Povidone-Iodine
  • Glutaral
  • Methanol