DAZ1/DAZ2 cluster deletion mediated by gr/gr recombination per se may not be sufficient for spermatogenesis impairment: a study of Chinese normozoospermic men

Asian J Androl. 2006 Mar;8(2):183-7. doi: 10.1111/j.1745-7262.2006.00121.x.

Abstract

Aim: To explore the possible effect of the deleted in azoospermia (DAZ) copy cluster deletion on spermatogenesis in the Chinese population, the deletion of the azoospermia factor c (AZFc) region was analyzed in 346 normozoospermic men.

Methods: Three DAZ single nucleotide variant loci and seven AZFc-specific sequence-tagged sites were examined with polymerase chain reaction (PCR)-restriction fragment length polymorphism and routine PCR.

Results: Five (1.4%) of the normozoospermic men were found to have deletion of gr/gr-DAZ1/DAZ2. None of the men were found to have b2/b4-entire DAZ deletion.

Conclusion: The presence of gr/gr-DAZ1/DAZ2 deletion in five men with normozoospermia suggests that this deletion per se may not be sufficient for spermatogenic impairment in Chinese men.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asian People / genetics
  • China
  • Chromosomes, Human, Y / genetics*
  • Gene Deletion*
  • Humans
  • Male
  • Oligospermia / genetics
  • Polymorphism, Restriction Fragment Length
  • RNA-Binding Proteins / genetics*
  • Recombination, Genetic / genetics
  • Sequence Tagged Sites
  • Spermatogenesis / genetics*

Substances

  • DAZAP1 protein, human
  • DAZAP2 protein, human
  • RNA-Binding Proteins