LCD-based detection of enzymatic action

Chem Commun (Camb). 2006 Jan 28:(4):434-5. doi: 10.1039/b514048j.

Abstract

By incorporating an ester-containing substrate in a self-assembled alignment layer for liquid crystal cells, the presence of a lipase (CALB) can be directly detected through its enzymatic action on the alignment layer, without the need for fluorescent labelling or enzyme assays.

MeSH terms

  • Biosensing Techniques / methods*
  • Candida / enzymology*
  • Enzyme Activation
  • Fungal Proteins
  • Hydrolysis
  • Lipase / chemistry
  • Lipase / metabolism*
  • Liquid Crystals / chemistry*
  • Microscopy, Electron, Transmission
  • Nanostructures
  • Propylamines
  • Silanes / chemistry
  • Substrate Specificity
  • Tin Compounds / chemistry

Substances

  • Fungal Proteins
  • Propylamines
  • Silanes
  • Tin Compounds
  • indium tin oxide
  • Lipase
  • lipase B, Candida antarctica
  • amino-propyl-triethoxysilane