Identification of antibodies against HAI-1 and integrin alpha6beta4 as immunohistochemical markers of human villous cytotrophoblast

J Histochem Cytochem. 2006 Jul;54(7):745-52. doi: 10.1369/jhc.5A6816.2006. Epub 2006 Feb 22.


Syncytiotrophoblast and invasive extravillous trophoblast arise from a common stem cell, namely villous cytotrophoblast, but have very different characteristics. The study of the differentiation process relies on the availability of suitable markers for these different cell types of developing placenta. In this work, we have produced monoclonal antibodies that are specific to human villous cytotrophoblast. Monoclonal antibody (MAb) MG2 was specific to villous cytotrophoblast across gestation, and recognizes hepatocyte growth factor activator inhibitor type 1. MAb MD10 stained villous cytotrophoblast across gestation and also some endothelial cells, particularly in the second or third trimester. MAb MD10 recognizes human integrin alpha6beta4. As a test for specificity, the novel MAbs were also used for staining of frozen tissue from human colon carcinoma. The results show that the two antibodies can be used as tools to study human villous cytotrophoblasts and also human tumors. The MG2 antibody seems most specific and promising for the study of various aspects of human villous cytotrophoblast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Biomarkers / metabolism
  • Cells, Cultured
  • Chorionic Villi / immunology*
  • Chorionic Villi / metabolism
  • Colonic Neoplasms / metabolism
  • Female
  • Humans
  • Immunohistochemistry
  • Immunoprecipitation
  • Integrin alpha6beta4 / immunology*
  • Integrin alpha6beta4 / metabolism
  • Membrane Glycoproteins / immunology*
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Pregnancy
  • Proteinase Inhibitory Proteins, Secretory
  • Trophoblasts / metabolism*


  • Antibodies, Monoclonal
  • Biomarkers
  • Integrin alpha6beta4
  • Membrane Glycoproteins
  • Proteinase Inhibitory Proteins, Secretory
  • SPINT1 protein, human