Overexpression of Stathmin in Oral Squamous-Cell Carcinoma: Correlation With Tumour Progression and Poor Prognosis

Br J Cancer. 2006 Mar 13;94(5):717-23. doi: 10.1038/sj.bjc.6602991.

Abstract

Stathmin is an intracellular phosphoprotein that is overexpressed in a number of human malignancies. Our previous study using proteomic profiling showed that significant upregulation of stathmin occurs in oral squamous-cell carcinoma (OSCC)-derived cell lines. In the current study, to determine the potential involvement of stathmin in OSCC, we evaluated the state of stathmin protein and mRNA expression in OSCC-derived cell lines and human primary OSCCs. A significant increase in stathmin expression was observed in all OSCC-derived cell lines examined compared to human normal oral keratinocytes. In immunohistochemistry, 65% of the OSCCs were positive for stathmin, and no immunoreaction was observed in corresponding normal tissues. Real-time quantitative reverse transcriptase-polymerase chain reaction data were consistent with the protein expression status. Moreover, stathmin expression status was correlated with the TNM stage grading. Furthermore, we found a statistical correlation between the protein expression status and disease-free survival (P=0.029). These results suggest that expression of stathmin could contribute to cancer progression/prognosis, and that stathmin may have potential as a biomarker and a therapeutic target for OSCC.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers, Tumor / analysis
  • Carcinoma, Squamous Cell / genetics*
  • Disease-Free Survival
  • Female
  • Gene Expression Profiling
  • Humans
  • Immunohistochemistry
  • Keratinocytes / physiology
  • Male
  • Middle Aged
  • Mouth Neoplasms / genetics*
  • Neoplasm Staging
  • Prognosis
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stathmin / biosynthesis*
  • Stathmin / genetics
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Biomarkers, Tumor
  • RNA, Messenger
  • STMN1 protein, human
  • Stathmin