Building mammalian signalling pathways with RNAi screens

Nat Rev Mol Cell Biol. 2006 Mar;7(3):177-87. doi: 10.1038/nrm1860.


Technological advances in mammalian systems are providing new tools to identify the molecular components of signalling pathways. Foremost among these tools is the ability to knock down gene function through the use of RNA interference (RNAi). The fact that RNAi can be scaled up for use in high-throughput techniques has motivated the creation of genome-wide RNAi reagents. We are now at the brink of being able to harness the power of RNAi for large-scale functional discovery in mammalian cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Mammals
  • Models, Biological
  • RNA Interference / physiology*
  • RNA, Viral / genetics
  • Signal Transduction / genetics*
  • Viruses / genetics


  • RNA, Viral