Conditional knockout mouse for tissue-specific disruption of the cyclooxygenase-2 (Cox-2) gene

Genesis. 2006 Mar;44(3):143-9. doi: 10.1002/gene.20192.

Abstract

Cyclooxygenase-2 (Cox-2) modulates many normal functions, and appears to play a role in a wide variety of pathophysiologic conditions. Cox-2 gene expression is induced in many different cell types, in response to many distinct stimuli. We generated a conditional knockout mouse in which critical exons of the Cox-2 gene are flanked with loxP sites. Cox-2(flox/flox) mice appear normal and are fertile. Recombination at the loxP sites, loss of Cox-2 protein expression, and prevention of induced PGE2 accumulation are observed in Cox-2(flox/flox) mouse embryo fibroblasts following infection with an adenovirus expressing CRE recombinase. In vivo recombination at the Cox-2(flox) allele was demonstrated in the liver of Cox-2(flox/flox) mice following intravenous injection of adenovirus expressing CRE recombinase. Spatially and temporally restricted elimination of the Cox-2 gene in Cox-2(flox/flox) conditional knockout mice should provide a valuable tool to analyze the cell type-specific role of Cox-2 in many disease models.

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Cyclooxygenase 2 / genetics*
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / enzymology
  • Female
  • Fibroblasts / cytology
  • Fibroblasts / enzymology
  • Gene Targeting*
  • Heterozygote
  • Homozygote
  • Integrases / metabolism*
  • Male
  • Mice
  • Mice, Knockout
  • Polymerase Chain Reaction
  • Prostaglandins / metabolism
  • Recombination, Genetic
  • Sequence Deletion
  • Transfection

Substances

  • Prostaglandins
  • Cyclooxygenase 2
  • Cre recombinase
  • Integrases