Protective effects of heat shock protein 70 induced by geranylgeranylacetone on oxidative injury in rat intestinal epithelial cells

Scand J Gastroenterol. 2006 Mar;41(3):312-7. doi: 10.1080/00365520500319427.

Abstract

Objective: Geranylgeranylacetone (GGA), an anti-ulcer agent, has recently been demonstrated to protect a variety of cells and tissues via induction of heat shock protein (HSP)70 against numerous stresses. We investigated whether GGA induces HSP70 and protects against an oxidative stressor, monocrolamine (NH(2)Cl), in a rat intestinal epithelial cell line (IEC-18).

Material and methods: IEC-18 cells pretreated with GGA (0.1-10 microM) were subjected to injury induced by NH(2)Cl. Cell viability was assessed, and endogenous HSP70 levels were determined by enzyme-linked immunosorbent assay in IEC-18 cells.

Results: Treatment with GGA (0.1-10 microM) was found rapidly to elevate HSP70 levels and to protect against NH(2)Cl-induced injury in IEC-18 cells. Furthermore, quercetin, an inhibitor of HSP70 synthesis, diminished the protective effects of GGA in IEC-18 cells upon NH(2)Cl-caused injury.

Conclusions: The results of this study suggest that GGA plays an important role in defense mechanisms against oxidative injury in the intestine, primarily via induction of HSP70.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Ulcer Agents / pharmacology*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chloramines / toxicity
  • Diterpenes / pharmacology*
  • Enzyme-Linked Immunosorbent Assay
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Epithelial Cells / pathology
  • HSP70 Heat-Shock Proteins / metabolism*
  • Humans
  • In Vitro Techniques
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism*
  • Intestinal Mucosa / pathology
  • Oxidative Stress / drug effects*
  • Rats

Substances

  • Anti-Ulcer Agents
  • Chloramines
  • Diterpenes
  • HSP70 Heat-Shock Proteins
  • chloramine
  • geranylgeranylacetone