Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry

J Mass Spectrom. 2006 Mar;41(3):372-89. doi: 10.1002/jms.997.


The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)2C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MS(n) analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry lends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Glycerophospholipids / analysis
  • Glycerophospholipids / chemistry
  • Glycosphingolipids / analysis
  • Glycosphingolipids / chemistry
  • Mass Spectrometry / methods*
  • Saccharomyces cerevisiae / chemistry*
  • Sphingolipids / analysis*
  • Sphingolipids / chemistry
  • Sphingosine / analogs & derivatives
  • Sphingosine / analysis
  • Sphingosine / chemistry


  • Glycerophospholipids
  • Glycosphingolipids
  • Sphingolipids
  • inositolphosphoceramides
  • mannosylinositol phosphoylceramide
  • Sphingosine
  • safingol