[Effect of protein kinase C on human melanoma A375-S2 cell death induced by evodiamine]

Yao Xue Xue Bao. 2005 Nov;40(11):1033-6.
[Article in Chinese]


Aim: To study the role of PKC in evodiamine-induced A375-S2 cell death.

Methods: Ratio of apoptosis induced by evodiamine was determined by TUNEL assay. MTT assay was carried out to assess cytotoxic effect of evodiamine. The influence on expression of ERK, phospho-ERK and Bcl-2 was detected by Western blotting analysis.

Results: TUNEL assay indicated that apoptosis was the type of A375-S2 cell death induced by evodiamine treatment for 24 h. Both staurosporine (inhibitor of PKC) and PD98059 (inhibitor of ERK) cooperated with evodiamine to further induce A375-S2 cell death. Evodiamine inhibited PKC activity, down-regulated the expression of ERK, phospho-ERK and Bcl-2, and staurosporine was capable of augmenting these effects induced by evodiamine.

Conclusion: PKC lies upstream and exhibits regulatory effect on ERK and Bcl-2 in evodiamine-induced cell death.

MeSH terms

  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Evodia / chemistry
  • Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Flavonoids / pharmacology
  • Humans
  • Melanoma / pathology
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plants, Medicinal / chemistry
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Quinazolines / isolation & purification
  • Quinazolines / pharmacology*
  • Staurosporine / pharmacology


  • Flavonoids
  • Plant Extracts
  • Proto-Oncogene Proteins c-bcl-2
  • Quinazolines
  • evodiamine
  • Protein Kinase C
  • Extracellular Signal-Regulated MAP Kinases
  • Staurosporine
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one