A clinical method for the determination of serum gamma-glutamyl transpeptidase

Clin Chim Acta. 1975 Nov 15;65(1):21-7. doi: 10.1016/0009-8981(75)90330-7.

Abstract

A simple, highly sensitive and reproducible method for the assay of gamma-glutamyl transpeptidase (EC 2.3.2-) activity is introduced, using gamma-glutamyl-p-nitroanilide as a substrate and glycylglycine as an acceptor in 50 g/l of polyoxyethylene nonylphenol. Serum transpeptidase activity was assayed in 1080 healthy adults, the normal mean value being 14.8 mU/ml. The diagnostic evaluation of the enzyme in various hepatobiliary diseases is also discussed.

MeSH terms

  • Adult
  • Biliary Tract Diseases / diagnosis
  • Detergents / pharmacology
  • Female
  • Humans
  • Liver Diseases / diagnosis
  • Male
  • Microchemistry
  • Spectrophotometry / methods
  • gamma-Glutamyltransferase / blood*
  • gamma-Glutamyltransferase / metabolism

Substances

  • Detergents
  • gamma-Glutamyltransferase