Identification and DNA Sequence of a New Bacteroides Fragilis Insertion Sequence-Like Element

Plasmid. 1991 Mar;25(2):141-4. doi: 10.1016/0147-619x(91)90027-t.


A new Bacteroides fragilis insertion sequence (IS)-like element has been identified, cloned, and sequenced. The element is 1598 base pairs in length. It is flanked by a 15-base pair imperfect inverted repeat and contains a large open reading frame which could encode a 430 amino acid protein. There is an 8-base pair duplication of genomic DNA sequences at the site of integration. One copy of the IS-like element is integrated within the 5' upstream sequence of the metallo-beta-lactamase gene ccrA, cloned from B. fragilis TAL3636. The IS-like element is integrated 19 bp upstream of the predicted initiation codon and, therefore, probably provides the transcriptional start signals for the CcrA gene.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacteroides fragilis / genetics*
  • Base Sequence
  • DNA Transposable Elements*
  • DNA, Bacterial / genetics*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • beta-Lactamases*


  • Bacterial Proteins
  • DNA Transposable Elements
  • DNA, Bacterial
  • beta-Lactamases
  • carbapenemase

Associated data

  • GENBANK/J03326
  • GENBANK/S43945
  • GENBANK/S43967
  • GENBANK/X54193
  • GENBANK/X54314
  • GENBANK/X55687
  • GENBANK/X55688
  • GENBANK/X55689
  • GENBANK/X55690
  • GENBANK/X56856
  • GENBANK/X58180