Mapping collagen organization in the human cornea: left and right eyes are structurally distinct

Invest Ophthalmol Vis Sci. 2006 Mar;47(3):901-8. doi: 10.1167/iovs.05-0893.


Purpose: Aspects of the biomechanics and surface topography of fellow human corneas are known to exhibit midline symmetry, but the structural basis of these observations is poorly understood. The mechanical performance of the cornea is strongly influenced by the organization of stromal collagen fibrils. The present study was designed to examine and compare the organization of collagen fibrils in the corneal stroma of left and right eyes.

Methods: Wide-angle x-ray scattering was used to map in detail the orientation and distribution of fibrillar collagen across the cornea, limbus, and adjacent sclera of three normal human eyes, including a fellow pair, and the central 9-mm corneal region of a further four eyes.

Results: Fibrillar collagen in the human cornea and limbus is arranged anisotropically, and in a highly specific manner. Left and right corneas are structurally distinct. In general, the mass distribution of preferentially aligned fibrils in the cornea appears to exhibit a degree of midline symmetry between left and right eyes.

Conclusions: Structural information, such as that presented herein, will enable a better understanding of corneal biomechanics and shape. Midline symmetry in the distribution of aligned, mechanically reinforcing collagen fibrils between left and right eyes may relate to the biomechanical and topographical enantiomorphism reported in the literature.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anisotropy
  • Biomechanical Phenomena
  • Corneal Stroma / metabolism*
  • Corneal Topography
  • Fibrillar Collagens / chemistry
  • Fibrillar Collagens / metabolism*
  • Functional Laterality
  • Humans
  • X-Ray Diffraction / methods


  • Fibrillar Collagens