Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation

Int Endod J. 2006 Mar;39(3):238-44. doi: 10.1111/j.1365-2591.2006.01080.x.


Aim: To examine whether low-power laser irradiation (LPLI) promotes cellular proliferation of human dental pulp-derived fibroblast-like cells (dental pulp cells).

Methodology: Dental pulp cells were obtained by primary culture of human dental pulp tissues from extracted third molar teeth. The phosphorylation of the mitogen-activated protein kinase (MAPK) family after LPLI of these cells was investigated by Western blotting. By using a specific MAPK/ERK kinase (MEK) inhibitor (PD098059), the specific effect of LPLI on the MAPK pathway was also investigated by Western blotting as described above. The incorporation of [3H]thymidine into the cells after LPLI was determined, and statistical analysis was performed by Wilcoxon signed-ranks test.

Results: Extracellular signal-regulated protein kinase (ERK) 1/2 was phosphorylated between 5 and 30 min after LPLI. Moreover, PD098059 inhibited LPLI-mediated ERK1/2 activation. LPLI did not affect p38 MAPK or c-Jun N-terminal kinase (JNK) phosphorylation. But LPLI did not stimulate [3H]thymidine incorporation into these cells.

Conclusions: These results indicated that LPLI activated MAPK/ERK, a signal for proliferation, differentiation and survival, but did not activate the stress signals p38 MAPK and JNK in human dental pulp cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dental Pulp / cytology
  • Dental Pulp / radiation effects*
  • Enzyme Activation / radiation effects
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Extracellular Signal-Regulated MAP Kinases / radiation effects*
  • Fibroblasts / radiation effects
  • Humans
  • Laser Therapy*
  • Mitogen-Activated Protein Kinases / metabolism
  • Mitogen-Activated Protein Kinases / radiation effects*
  • Statistics, Nonparametric


  • Extracellular Signal-Regulated MAP Kinases
  • Mitogen-Activated Protein Kinases