Nucleotide sequence of IS402 from Pseudomonas cepacia

Gene. 1991 Jun 15;102(1):143-4. doi: 10.1016/0378-1119(91)90555-p.

Abstract

IS402, a transposable gene-activating element isolated on the basis of its ability to increase expression of the Tn1 bla gene in Pseudomonas cepacia, was cloned from pTGL52 into the vector, pBluescript KS+, and its nucleotide (nt) sequence was determined. This 914-bp element had terminal inverted repeats of 17 bp with a single mismatch, and upon insertion into Tn1 generated a direct target duplication of 3 bp. Comparison of its nt sequence with the GenBank and EMBL databases indicated that IS402 is unrelated to previously described bacterial IS elements.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA Transposable Elements / genetics*
  • Molecular Sequence Data
  • Pseudomonas / genetics*
  • Repetitive Sequences, Nucleic Acid / genetics

Substances

  • DNA Transposable Elements

Associated data

  • GENBANK/M33173
  • GENBANK/S39290
  • GENBANK/S39297
  • GENBANK/S46113
  • GENBANK/S47661
  • GENBANK/S70115
  • GENBANK/S70117
  • GENBANK/S70121
  • GENBANK/S70125
  • GENBANK/S70128
  • GENBANK/S70130