Effects of 1,25-dihydroxy-vitamin D3 on phosphate accumulation by myoblasts

Horm Metab Res. 1991 Mar;23(3):113-6.


The effects of 1,25-dihydroxy-Vitamin D3 on phosphate uptake by cultured chick embryonic muscle cells were investigated. Preincubation of primary myoblast cultures during 4-24 hours with physiological levels of 1,25(OH)2D3 resulted in a significant stimulation of velocity and total capacity of phosphate accumulation by the cells. Maximal responses were obtained at 8 hours of treatment with the sterol. In agreement with previous studies, 25-hydroxy-Vitamin D3 also stimulated myoblast phosphate uptake. 24,25-dihydroxy-Vitamin D3 and vitamin D3 were ineffective. Evidence was obtained indicating that 1,25(OH)2D3 affects the Na(+)-linked component of muscle cell phosphate uptake through a mechanism dependent on "de movo" protein and RNA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Calcifediol / pharmacology
  • Calcitriol / pharmacology*
  • Cells, Cultured
  • Chick Embryo
  • Cholecalciferol / pharmacology
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Kinetics
  • Muscles / drug effects
  • Muscles / metabolism*
  • Phosphates / metabolism*
  • Phosphorus Radioisotopes
  • Sodium / pharmacology


  • Phosphates
  • Phosphorus Radioisotopes
  • Cholecalciferol
  • Dactinomycin
  • Cycloheximide
  • Sodium
  • Calcitriol
  • Calcifediol