Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I

Galina S Kachalova; Eugeny A Rogulin; Rimma I Artyukh; Tatyana A Perevyazova; Ludmila A Zheleznaya; Nickolay I Matvienko; Hans D Bartunik

doi:10.1107/S174430910500309X

Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Mar 1;61(Pt 3):332-4. doi: 10.1107/S174430910500309X. Epub 2005 Mar 1.

Authors

Galina S Kachalova¹, Eugeny A Rogulin, Rimma I Artyukh, Tatyana A Perevyazova, Ludmila A Zheleznaya, Nickolay I Matvienko, Hans D Bartunik

Affiliation

¹ Max-Planck Research Unit for Structural Biology, Protein Dynamics Group, Hamburg 22607, Germany. galina@mpghdb.desy.de

Abstract

Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / enzymology
Bacterial Proteins / chemistry
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism
Crystallization
Crystallography, X-Ray
Deoxyribonuclease I / chemistry
Deoxyribonuclease I / isolation & purification
Deoxyribonuclease I / metabolism*
Kinetics
Substrate Specificity
X-Ray Diffraction

Substances

Bacterial Proteins
Deoxyribonuclease I