Crystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt 2):97-9. doi: 10.1107/S1744309105041011. Epub 2006 Jan 27.

Abstract

The opportunistic yeast Malassezia sympodialis can act as an allergen and elicit specific IgE- and T-cell reactivity in patients with atopic eczema. The first identified major allergen from M. sympodialis, Mala s 1, is present on the cell surface of the yeast. Recombinant Mala s 1 was expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Mala s 1 were obtained in 25% PEG 8K, 0.2 M (NH4)2SO4. Crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 44.4, b = 163.7, c = 50.6 A, and diffract to 1.35 A resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / chemistry*
  • Allergens / immunology
  • Allergens / isolation & purification
  • Antigens, Fungal / chemistry*
  • Antigens, Fungal / immunology
  • Antigens, Fungal / isolation & purification
  • Crystallization
  • Crystallography, X-Ray
  • Fungal Proteins / chemistry*
  • Fungal Proteins / immunology
  • Fungal Proteins / isolation & purification
  • Humans
  • Malassezia* / immunology

Substances

  • Allergens
  • Antigens, Fungal
  • Fungal Proteins
  • Mala s 1 allergen, Malassezia sympodialis