Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt 2):163-5. doi: 10.1107/S1744309106001448. Epub 2006 Jan 27.

Abstract

The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 A, alpha = 89.92, beta = 76.01, gamma = 76.99 degrees. X-ray diffraction data to a resolution of 3.0 A have been collected under cryoconditions (100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose-specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Dolichos / chemistry*
  • Dolichos / metabolism
  • Galactose / chemistry
  • Galactose / metabolism*
  • Glycine max / chemistry
  • Plant Lectins / chemistry*
  • Plant Lectins / metabolism*
  • Protein Binding
  • Seeds / chemistry
  • Seeds / metabolism
  • Soybean Proteins / chemistry

Substances

  • Plant Lectins
  • Soybean Proteins
  • soybean lectin
  • Galactose