Feedback control of mitosis in budding yeast

Cell. 1991 Aug 9;66(3):519-31. doi: 10.1016/0092-8674(81)90015-5.


We have investigated the feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. We isolated budding yeast mutants sensitive to the anti-microtubule drug benomyl. Mitotic arrest-deficient (mad) mutants are the subclass of benomyl-sensitive mutants in which the completion of mitosis is not delayed in the presence of benomyl and that die as a consequence of their premature exit from mitosis. A number of properties of the mad mutants indicate that they are defective in the feedback control over the exit from mitosis: their killing by benomyl requires passage through mitosis; their benomyl sensitivity can be suppressed by an independent method for delaying the exit from mitosis; they have normal microtubules; and they have increased frequencies of chromosome loss. We cloned MAD2, which encodes a putative calcium-binding protein whose disruption is lethal. We discuss the role of feedback controls in coordinating events in the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Benomyl / pharmacology
  • Calcium-Binding Proteins / genetics*
  • Carrier Proteins*
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Nucleus / physiology
  • Chromosomes, Fungal / metabolism
  • Cloning, Molecular
  • Cytoskeleton / physiology
  • DNA Mutational Analysis
  • DNA, Fungal / genetics
  • Feedback
  • Fungal Proteins / genetics*
  • Genes, Fungal
  • Mad2 Proteins
  • Microtubules / physiology*
  • Mitosis*
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins
  • Protamine Kinase / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae Proteins
  • Spindle Apparatus / drug effects
  • Spindle Apparatus / physiology*


  • Calcium-Binding Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA, Fungal
  • Fungal Proteins
  • MAD2 protein, S cerevisiae
  • Mad2 Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Protamine Kinase
  • Benomyl

Associated data

  • GENBANK/M60962
  • GENBANK/M64383
  • GENBANK/M64384
  • GENBANK/M64707
  • GENBANK/M73821
  • GENBANK/S44812
  • GENBANK/S44816
  • GENBANK/S44821
  • GENBANK/S56137
  • GENBANK/S56139
  • GENBANK/U14132