A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR

Cell. 1991 Aug 9;66(3):555-61. doi: 10.1016/0092-8674(81)90018-0.


The vitamin A derivative retinoic acid exerts its effects on transcription through two distinct classes of nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). We provide evidence that expression of the gene for cellular retinol-binding protein type II (CRBPII), a key protein in the intestinal absorption of vitamin A, is dramatically up-regulated by retinoic acid in the presence of RXR but not RAR. This regulation is conferred through a specific cis element in the CRBPII promoter that contains five nearly perfect tandem repeats of the sequence AGGTCA spaced by a single nucleotide. The discovery of this new RX response element provides a means for distinguishing between the two retinoid receptor systems and suggests that an RXR-mediated pathway exists for modulating vitamin A metabolism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carrier Proteins / physiology*
  • Cell Line
  • DNA Mutational Analysis
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation* / drug effects
  • Genes
  • In Vitro Techniques
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Promoter Regions, Genetic*
  • Protein Binding
  • RNA, Messenger / genetics
  • Receptors, Retinoic Acid
  • Regulatory Sequences, Nucleic Acid
  • Retinol-Binding Proteins / genetics*
  • Retinol-Binding Proteins, Cellular
  • Transcription Factors / physiology*
  • Transcription, Genetic
  • Tretinoin / pharmacology


  • Carrier Proteins
  • DNA-Binding Proteins
  • Oligonucleotides
  • RNA, Messenger
  • Receptors, Retinoic Acid
  • Retinol-Binding Proteins
  • Retinol-Binding Proteins, Cellular
  • Transcription Factors
  • Tretinoin