Abstract
An increased activity of ATP-binding cassette transporter (ABC) A1 has been proposed as a mechanism underlying the hypocholesterolaemic effect of phytosterols. In the present study, ABCA1-deficient mice (ABCA1-/- mice) were used to examine the involvement of the ABCA1 in the reduction of intestinal cholesterol absorption in response to a phytosterol-enriched diet. A decrease in intestinal cholesterol absorption of 39 and 35 % was observed after phytosterol treatment in ABCA1+/+ mice and in ABCA1-/- mice, respectively. No statistically significant changes in plasma lipoprotein profile or in intestinal ABCG5, ABCG8 and Niemann-Pick C1-Like 1 gene expression levels were found when phytosterol-treated ABCA1-/- mice and untreated ABCA1-/- mice were compared. We conclude that phytosterol inhibition of cholesterol absorption in mice is independent of ABCA1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter 1
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ATP Binding Cassette Transporter, Subfamily G, Member 5
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ATP Binding Cassette Transporter, Subfamily G, Member 8
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ATP-Binding Cassette Transporters / analysis
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ATP-Binding Cassette Transporters / metabolism*
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Absorption
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Animals
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Cholesterol, Dietary / pharmacokinetics*
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Dietary Supplements*
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Gene Expression
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Intestinal Mucosa / metabolism*
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Lipoproteins / analysis
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Membrane Transport Proteins / analysis
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Mice
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Mice, Inbred DBA
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Phytosterols / administration & dosage*
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Reverse Transcriptase Polymerase Chain Reaction / methods
Substances
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ABCG5 protein, mouse
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ABCG8 protein, mouse
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ATP Binding Cassette Transporter 1
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ATP Binding Cassette Transporter, Subfamily G, Member 5
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ATP Binding Cassette Transporter, Subfamily G, Member 8
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ATP-Binding Cassette Transporters
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Cholesterol, Dietary
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Lipoproteins
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Membrane Transport Proteins
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Npc1l1 protein, mouse
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Phytosterols