In vitro effects of vitamin C and selenium on NK activity of patients with beta-thalassemia major

Pediatr Hematol Oncol. Apr-May 2006;23(3):187-97. doi: 10.1080/08880010500506420.


In this study, the in vitro effects of vitamin C and selenium on natural killer (NK) cell activity of ss-thalassemia major patients was investigated. At first, significant decreased NK activity was found at E:T ratios of 1:1, 5:1, and 10:1 in whole thalassemia patients. Low-dose selenium treatment enhanced NK activity in patients but there was no change in the control group. High-dose selenium decreased NK activity significantly in splenectomized patients. Different doses vitamin C enhanced NK activity significantly in both splenectomized and unsplenectomized patients. According to these results, selenium dosage should be arranged carefully in thalassemia patients, whereas vitamin C can be used confidently.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antioxidants / pharmacology*
  • Ascorbic Acid / administration & dosage
  • Ascorbic Acid / pharmacology*
  • Cells, Cultured / drug effects
  • Child
  • Cytotoxicity Tests, Immunologic
  • Cytotoxicity, Immunologic / drug effects
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical
  • Female
  • Humans
  • K562 Cells
  • Killer Cells, Natural / drug effects*
  • Male
  • Selenium / administration & dosage
  • Selenium / pharmacology*
  • Splenectomy
  • beta-Thalassemia / blood
  • beta-Thalassemia / immunology*
  • beta-Thalassemia / surgery


  • Antioxidants
  • Selenium
  • Ascorbic Acid