Effects of p-chloroamphetamine on release of [3H]gamma-aminobutyric acid from slices of rat caudate-putamen

Eur J Pharmacol. 1991 Apr 17;196(2):189-95. doi: 10.1016/0014-2999(91)90427-r.

Abstract

The effect of endogenous serotonin on the release of [3H]gamma-aminobutyric acid ([3H]GABA) from slices of rat caudate-putamen was studied. p-Chloroamphetamine was used to release endogenous serotonin. p-Chloroamphetamine (100 nM) enhanced the release of [3H]GABA induced by 20 mM K+, while 1000 nM p-chloroamphetamine decreased it. The 5-HT3 receptor antagonists ICS 205-930 (50 nM) and MDL 72222 (100 nM) prevented this facilitation caused by 100 nM p-chloroamphetamine. ICS 205-903 (50 nM), when used alone, reduced the release of [3H]GABA caused by 23 mM K+. This finding confirmed the hypothesis that endogenous serotonin can enhance the release of [3H]GABA via 5-HT3 receptors. In contrast, an effect of 5-HT1 and 5-HT2 receptors could not be clearly established. It is likely that the release of endogenous GABA from striatonigral GABA neurons may also be affected by serotonin via 5-HT3 receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caudate Nucleus / drug effects*
  • Caudate Nucleus / metabolism
  • In Vitro Techniques
  • Indoles / pharmacology
  • Male
  • Putamen / drug effects*
  • Putamen / metabolism
  • Rats
  • Rats, Inbred Strains
  • Receptors, Adrenergic, alpha / drug effects
  • Receptors, Adrenergic, alpha / physiology
  • Receptors, Serotonin / drug effects
  • Receptors, Serotonin / physiology
  • Serotonin Antagonists / pharmacology
  • Tropanes / pharmacology
  • Tropisetron
  • gamma-Aminobutyric Acid / metabolism*
  • p-Chloroamphetamine / pharmacology*

Substances

  • Indoles
  • Receptors, Adrenergic, alpha
  • Receptors, Serotonin
  • Serotonin Antagonists
  • Tropanes
  • gamma-Aminobutyric Acid
  • p-Chloroamphetamine
  • Tropisetron
  • bemesetron