Primary structure and functional characterization of a human 5-HT1D-type serotonin receptor

Mol Pharmacol. 1991 Aug;40(2):143-8.


We describe the nucleic acid sequence encoding a human 5-hydroxytryptamine1D (5-HT1D) serotonin receptor and some of the functional characteristics of the gene product. The receptor gene was isolated by hybridization to a probe based on a canine thyroid cDNA (called RDC4) previously isolated by others and believed to encode a heretofore undetermined member of the guanine nucleotide-binding protein (G protein)-linked receptor family. The human clone we isolated, called MA6A, contains an apparently intronless open reading frame encoding a 377-amino acid polypeptide with the seven hydrophobic domains characteristic of G protein-linked receptors. The MA6A deduced amino acid sequence is 88% identical to that for RDC4 and 43% identical to that for the human 5-HT1A receptor. Expression of the human gene product in transfected cell lines results in the appearance of saturable high affinity 5-HT1D-type [3H]5-HT binding. The expressed receptor exhibits features indicative of coupling to Gi proteins, i.e., robust inhibition of forskolin-stimulated cAMP accumulation and formation of a pertussis toxin-sensitive high agonist affinity binding state. These findings may help clarify several ambiguities in the classification and action of serotonin receptor subtypes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cyclic AMP / metabolism
  • GTP-Binding Proteins / analysis
  • Humans
  • Molecular Sequence Data
  • Pertussis Toxin
  • Receptors, Serotonin / chemistry
  • Receptors, Serotonin / genetics*
  • Receptors, Serotonin / physiology
  • Virulence Factors, Bordetella / pharmacology


  • Receptors, Serotonin
  • Virulence Factors, Bordetella
  • Cyclic AMP
  • Pertussis Toxin
  • GTP-Binding Proteins

Associated data

  • GENBANK/M89955
  • GENBANK/X52371
  • GENBANK/X52372
  • GENBANK/X57181
  • GENBANK/X57182
  • GENBANK/X57183
  • GENBANK/X57184
  • GENBANK/X61717
  • GENBANK/X61718
  • GENBANK/X61719