Molecular characterization of the murine homologue of the DC-derived protein DC-SCRIPT

J Leukoc Biol. 2006 May;79(5):1083-91. doi: 10.1189/jlb.1005588. Epub 2006 Mar 7.


Dendritic cell-specific transcript (DC-SCRIPT) is a putative DC zinc (Zn) finger-type transcription factor described recently in humans. Here, we illustrate that DC-SCRIPT is highly conserved in evolution and report the initial characterization of the murine ortholog of DC-SCRIPT, which is also preferentially expressed in DC as shown by real-time quantitative polymerase chain reaction, and its distribution resembles that of its human counterpart. Studies undertaken in human embryonic kidney 293 cells depict its nuclear localization and reveal that the Zn finger domain of the protein is mainly responsible for nuclear import. The human and the mouse genes are located in syntenic chromosomal regions and exhibit a similar genomic organization with numerous common transcription factor-binding sites in their promoter region, including sites for many factors implicated in haematopoiesis and DC biology, such as Gfi, GATA-1, Spi-B, and c-Rel. Taken together, these data show that DC-SCRIPT is well-conserved in evolution and that the mouse homologue is more than 80% homologous to the human protein. Therefore, mouse models can be used to elucidate the function of this novel DC marker.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / physiology
  • Animals
  • Animals, Newborn
  • Binding Sites / genetics
  • Carrier Proteins
  • Cell Line
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human, Pair 5 / genetics
  • Conserved Sequence
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic / genetics
  • Protein Binding / genetics
  • Repressor Proteins / chemistry*
  • Repressor Proteins / genetics
  • Repressor Proteins / isolation & purification
  • Repressor Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Zinc Fingers / physiology


  • Carrier Proteins
  • DC-SCRIPT protein, mouse
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Repressor Proteins
  • Transcription Factors
  • ZNF366 protein, human