IL-10 genotype predicts serum levels of adhesion molecules, inflammation and atherosclerosis in hemodialysis patients

J Nephrol. Jan-Feb 2006;19(1):50-6.

Abstract

Background: Hemodialysis (HD) patients suffer from inflammation and accelerated atherosclerosis that accounts for a large number of premature deaths. The anti-inflammatory interleukin (IL)-10 gene is polymorphic and potential direct effects of IL-10 gene polymorphisms on the circulating serum levels of adhesion molecules, inflammation and atherosclerosis in HD patients still have to be elucidated.

Methods: In a cross-sectional study, circulating serum levels of vascular cell adhesion molecule-1 (VCAM-1), E-selectin (E-selectin), and intracellular adhesion molecule-1 (ICAM-1), serum albumin and C-reactive protein (CRP) were measured in 121 HD patients (70 male and 51 female, with mean age 49 +/- 18 yrs). Carotid artery intima media thickness (IMT) was evaluated by Doppler ultrasonography. Patients were genotyped for the polymorphic base at position -1082 of the IL-10 promoter sequence by polymerase chain reaction (PCR).

Results: Circulating serum levels of all three adhesion molecules were higher in patients with -1082/AA genotype (p = 0.001). Higher serum albumin levels together with lower CRP levels were observed in patients with -1082/GG genotype (p < 0.05). Prevalence of atherosclerosis was higher in patients with -1082/AA genotype compared to heterozygous and -1082/GG genotype (41, 34 and 26%, respectively, p = 0.018).

Conclusions: These results suggest that IL-10 gene polymorphism has an effect on inflammatory process and atherosclerosis in HD patients. Endothelial protective functions of IL-10 can modulate the circulating serum levels of adhesion molecules. Therefore, IL-10 gene polymorphism could lead to high or low inflammatory process and consequently, to atherosclerosis. IL-10 genotyping can define a high-risk group for atherosclerosis among HD patients.

Publication types

  • Comparative Study

MeSH terms

  • Atherosclerosis / blood*
  • Atherosclerosis / complications
  • Atherosclerosis / genetics
  • Blood Flow Velocity
  • Carotid Arteries / diagnostic imaging
  • Carotid Arteries / physiopathology
  • Cell Adhesion Molecules / blood*
  • DNA / genetics*
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genotype
  • Humans
  • Inflammation / blood
  • Inflammation / complications
  • Interleukin-10 / blood
  • Interleukin-10 / genetics*
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / complications
  • Kidney Failure, Chronic / therapy*
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Renal Dialysis*
  • Ultrasonography, Doppler

Substances

  • Cell Adhesion Molecules
  • Interleukin-10
  • DNA