Cloning and expression of human endothelin-1 receptor cDNA

FEBS Lett. 1991 Aug 5;287(1-2):23-6. doi: 10.1016/0014-5793(91)80007-p.


We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 greater than or equal to ET-2 much greater than ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 x 10(-7) M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 x 10(-7) M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • Blotting, Northern
  • Cell Line
  • Cloning, Molecular*
  • DNA / genetics*
  • Electrophysiology
  • Endothelins / metabolism
  • Endothelins / pharmacology
  • Gene Expression*
  • Humans
  • Molecular Sequence Data
  • Oocytes / physiology
  • Placenta / chemistry
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, Endothelin
  • Transfection
  • Xenopus


  • Endothelins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Endothelin
  • DNA