From Gene Expression Analysis to Tissue Microarrays: A Rational Approach to Identify Therapeutic and Diagnostic Targets in Lymphoid Malignancies

Mol Cell Proteomics. 2006 Jun;5(6):1072-81. doi: 10.1074/mcp.M600077-MCP200. Epub 2006 Mar 8.

Abstract

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Neoplasm
  • Case-Control Studies
  • Child
  • Epitopes
  • Epitopes, B-Lymphocyte
  • Humans
  • Lymphoid Tissue / chemistry
  • Lymphoid Tissue / cytology
  • Lymphoma, Mantle-Cell / chemistry*
  • Lymphoma, Mantle-Cell / genetics*
  • Lymphoma, Mantle-Cell / pathology
  • Lymphoma, Non-Hodgkin / chemistry*
  • Neoplasm Proteins / analysis*
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology
  • Oligonucleotide Array Sequence Analysis*
  • Proteomics / methods*
  • Tissue Array Analysis*

Substances

  • Antibodies, Neoplasm
  • Epitopes
  • Epitopes, B-Lymphocyte
  • Neoplasm Proteins