Characterization of the snowy cotyledon 1 mutant of Arabidopsis thaliana: the impact of chloroplast elongation factor G on chloroplast development and plant vitality

Plant Mol Biol. 2006 Mar;60(4):507-18. doi: 10.1007/s11103-005-4921-0.

Abstract

During seedling development chloroplast formation marks the transition from heterotrophic to autotrophic growth. The development and activity of chloroplasts may differ in cotyledons that initially serve as a storage organ and true leaves whose primary function is photosynthesis. A genetic screen was used for the identification of genes that affect selectively chloroplast function in cotyledons of Arabidopsis thaliana. Several mutants exhibiting pale cotyledons and green true leaves were isolated and dubbed snowy cotyledon (sco). One of the mutants, sco1, was characterized in more detail. The mutated gene was identified using map-based cloning. The mutant contains a point mutation in a gene encoding the chloroplast elongation factor G, leading to an amino acid exchange within the predicted 70S ribosome-binding domain. The mutation results in a delay in the onset of germination. At this early developmental stage embryos still contain undifferentiated proplastids, whose proper function seems necessary for seed germination. In light-grown sco1 seedlings the greening of cotyledons is severely impaired, whereas the following true leaves develop normally as in wild-type plants. Despite this apparent similarity of chloroplast development in true leaves of mutant and wild-type plants various aspects of mature plant development are also affected by the sco1 mutation such as the onset of flowering, the growth rate, and seed production. The onset of senescence in the mutant and the wild-type plants occurs, however, at the same time, suggesting that in the mutant this particular developmental step does not seem to suffer from reduced protein translation efficiency in chloroplasts.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / isolation & purification
  • Arabidopsis Proteins / metabolism*
  • Chlorophyll / metabolism
  • Chloroplasts / genetics
  • Chloroplasts / metabolism
  • Chloroplasts / physiology*
  • Chromosomes, Plant / genetics
  • Germination / physiology
  • Molecular Sequence Data
  • Mutation / genetics*
  • Peptide Elongation Factor G / metabolism*
  • Protein Transport
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Seedlings / growth & development
  • Seeds / physiology
  • Time Factors

Substances

  • Arabidopsis Proteins
  • Peptide Elongation Factor G
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • snowy cotyledon 1 protein, Arabidopsis
  • Chlorophyll
  • Ribulose-Bisphosphate Carboxylase