Liquid Tissue: proteomic profiling of formalin-fixed tissues

Biotechniques. 2005 Jun:Suppl:32-5. doi: 10.2144/05386su06.


Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue MS protein preparation allows researchers to utilize the vast, already existing, collections offormalin-fixed paraffin-embedded (FFPE) tissues for the procurement of peptides and the analysis across a variety of MS platforms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid
  • Colonic Neoplasms / chemistry*
  • Colonic Neoplasms / pathology
  • Formaldehyde / chemistry*
  • Humans
  • Mass Spectrometry
  • Neoplasm Proteins / analysis*
  • Paraffin Embedding
  • Proteomics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tissue Fixation*


  • Neoplasm Proteins
  • Formaldehyde