Inhibition of mouse T-cell proliferation by CGRP and VIP: effects of these neuropeptides on IL-2 production and cAMP synthesis

J Neurosci Res. 1991 May;29(1):29-41. doi: 10.1002/jnr.490290104.


We compared the effect of two neuropeptides, calcitonin gene-related peptide (CGRP) and vasoactive intestinal peptide (VIP), on mitogen-induced murine splenocyte proliferation. Both neuropeptides exerted their maximal effect within 24 hr after activation by Con A. The combination CGRP-VIP caused an additive inhibitory effect on T-cell proliferation. The inhibitory effect of VIP could be correlated with a decrease in interleukin 2 (IL-2) production, whereas CGRP did not affect this production. Since we also observed an additive inhibitory effect on T-cell proliferation by the theophylline and CGRP or VIP combination, we measured the effect of each neuropeptide on intracellular cAMP production by enriched T-cells: CGRP, but not VIP, strongly stimulated cAMP synthesis. Taken together, our results indicate that inhibition of murine T-cell proliferation by CGRP and VIP is mediated by different mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Cell Division / drug effects
  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Cyclic AMP / biosynthesis*
  • Female
  • In Vitro Techniques
  • Interleukin-2 / biosynthesis*
  • Mice
  • Mice, Inbred BALB C
  • Mitogens / pharmacology
  • Spleen / cytology
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / metabolism
  • Theophylline / pharmacology
  • Vasoactive Intestinal Peptide / pharmacology*


  • Interleukin-2
  • Mitogens
  • Concanavalin A
  • Vasoactive Intestinal Peptide
  • Theophylline
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide