Structure and expression of rat S-100 beta subunit gene

Brain Res Mol Brain Res. 1991 Jun;10(3):193-202. doi: 10.1016/0169-328x(91)90061-2.

Abstract

The gene structure for S-100 beta subunit has been elucidated. The gene spans about 8 kbp and consists of 3 exons and 2 introns. The transcription initiation site was determined by an S1 nuclease mapping. The promoter region contains TATA-box-like and CAAT-box-like sequences. To examine the activity of the promoter sequence, a transfection of pS100 beta-lacZ fused gene to the cultured cells was carried out. C6 glioma cells showed a positive expression of beta-galactosidase. Gene-deletion experiments suggested the functional importance of the DNA fragment (22 bp) containing TATA-box-like and CAAT-box-like sequences. A factor protein that binds to the 100 bp DNA fragment containing the promoter sequence was specifically detected in the rat brain nuclear extract.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain / physiology*
  • Cell Nucleus / physiology
  • Exons
  • Gene Expression
  • Gene Library
  • Genes*
  • HeLa Cells / physiology
  • Humans
  • Introns
  • Liver / physiology
  • Macromolecular Substances
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Rats
  • Restriction Mapping
  • S100 Proteins / genetics*
  • TATA Box
  • Transcription, Genetic

Substances

  • Macromolecular Substances
  • RNA, Messenger
  • S100 Proteins

Associated data

  • GENBANK/M60710
  • GENBANK/M60711
  • GENBANK/M60712
  • GENBANK/M60713
  • GENBANK/M60714
  • GENBANK/M60715
  • GENBANK/M60716
  • GENBANK/S53517
  • GENBANK/S53522
  • GENBANK/S53527